High Performance Thin Layer Chromatographic Quantification Domperidone and Paracetamol in Tablets Forms Domperidone, Paractamol, Silica gel HPTLC Plate, HPTL

A simple, precise, rapid, selective, (HPTLC) method has been established for simultaneous analysis of Domperidone (DMP) and paractamol (PAR) in tablet dosage forms. The Chromatographic separation was performed on per coated silica gel 60 GF254 .plate with acetone -methanolcarbon tetrachloride 5:5:1(V/V) as mobile phase. The plates were developed to a distance of 8.0 cm at ambient temperature. The developed plate were scanned and quantified at their single wavelength of maximum absorption at approximately 280 and 245nm for Domperidone and paractamol, respectively. Experimental conditions such as band size, chamber saturation time, migration of solvent front, slit width, etc .were critically studied and the optimum condition were selected .The drugs were satisfactorily resolved with Rf 0.53±0.02 for domperidone and 0.73± 0.02 for paracetamol. The method was validated for linearity, accuracy, precision and specificity. The calibration plot was linear between 15-45 ng per band for DMP and 850-2500 ng per band for PAR. The limit of detection and quantification for DMP were 0.23 and 0.189 ng per band. respectively ;forPAR they were 0.308 and0.938 ng per band. This HPTLC procedure is economic, sensitive, and less time consuming than other Chromatographic procedures. It is a user –friendly and important tool for analysis of combined tablets forms.